Purpose: To identify the purity of analgesics/ prescription drugs using TLC.
Introduction: Drug identification is determined by TLC, which is a analytical technique used to analyze the purity of chemical compounds.
Materials and Method: An unknown sample labeled number 2 and 7 (1% solution) was prepared in ethyl acetate and acetic acid . Iodine and a UV lamp were used to identify the spots of the samples. The TLC plates were made of Si and cut into 25x5 cm plates. The plates were placed inside a beaker containing filter paper and a watch glass was placed on top.
Procedure: A 1cm line was drawn at one end of the TLC plate and at the other end a 1in line was drawn. Above the 1cm line a capillary tube was used to spot the sample on to the plate. Once the sample was applied, the plate was placed into the beaker until the solvent reached the 1in line. The plate was removed from the beaker and placed on to a Kimwipe to dry. When the plate dried the samples 2 and 7 were viewed using a UV lamp and/or iodine.
Results: The Rf values were determine from figure 1. The Rf value for sample 2 was 0.50 and sample 7 value was 0.53. 
Figure 1. TLC Samples 2 and 7
Discussion: This experiment allowed the determination of purity of samples 2 and 7. The results determined that sample 2 spotting resulted in a streak. Sample 2 streaking was due to an overload or many components ran together creating a streak. Sample 7 resulted in a pure substance since there was no abnormality to the spot.
By: Alice Story
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