Objective: To learn the structure and properties of organic molecules.
Introduction: In Crime Scene Investigations, delicate instruments such as an ultraviolet lamp and TLC plates are used to analyze the the purity of chemical compounds: drugs and organic poisons. In this lab course, we used these instruments to determine the purity of analegesics found in traditional phramacies.
Materials and Methods: We weighed 1 mg of Sample 3 and Sample 6 into a valve a
nd mixed it with ethyl acetate and acetic acid. We drew a line 1 cm from the bottom of the verticle TLC plate with a pencil. Using a capillary tube, placed a few spots on the the TLC plate. Place a filter paper in a 400 mL beaker with the solvent. Once the TLC plate dries, placed it in the solvent of ethanol and acetic acid until the product diffuses 1 inch from the top. Observe data and record results using the UV lamp for detailed observations. To make results more visible, we used iodide in a beaker as seen in Figure 1.1.
nd mixed it with ethyl acetate and acetic acid. We drew a line 1 cm from the bottom of the verticle TLC plate with a pencil. Using a capillary tube, placed a few spots on the the TLC plate. Place a filter paper in a 400 mL beaker with the solvent. Once the TLC plate dries, placed it in the solvent of ethanol and acetic acid until the product diffuses 1 inch from the top. Observe data and record results using the UV lamp for detailed observations. To make results more visible, we used iodide in a beaker as seen in Figure 1.1.Results and Discussion: Performing a TLC analysis of the unknown analgesics, the number of components that were observed were 3 out of 3 in Sample 3 and 3 out of 3 in Sample 6. The results in Sample 3 were more faint compared to the results in Sample 6 as seen in Figure 1.2.
The R(f) value was 0.55 cm in each sample.
Conclusion: The purpose of this procedure was to learn how to determine the purity of traditional drugs similar to the methods that the CSI use when determining the purity of poisons and illegal drugs since many chemical compound are similar. In Sample 6, the spots are in a linear pattern as they were when the experiement began. Therefore, the drug in Sample 6 is pure. Because of the amount of uncertainiy in Sample 3 (due to lack of a higher magnification), the assumption is that this drug was also pure. The observations were faint but the trace up the TLC plate was not. Within this experiment, we learned the how to determine the purity of a substance using instruments that the CSI use.
by: Kanieshia N.
by: Kanieshia N.
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